Laser Micro-Dissection (LMD) & Genetic Analyses in New Preclinical Models to improve Pancreatic Cancer Treatment

Leica Laser Microdissection and Applications
From Eye to Insight

In this Webinar, after a technical presentation by Dr. Tamara Straube, Dr. Giovannetti discuss the advantages of using LMD techniques for precise, contamination-free isolation of specific cell types in various applications, such as using pancreatic cancer tissue sections from patients and orthotopic xenografts. Thus, this webinar provides an overview of the scientific and practical considerations for obtaining highly pure material for molecular analysis in the field of pharmacological studies to overcome key mechanisms of resistance in pancreatic cancer.

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Aula Abierta: Liquid Biopsies

Talking about liquid biopsies, and how this type of analysis can be used for.
– Leticia G. Leon

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Aula Abierta: Discussing real options to work as a researcher in Spain

Talking about the real possibilities to work in Spain as a researcher. The discussion presented different points of view. I explained why I had to move to another country to keep working in research.
– Leticia G. Leon

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Video ecografia sui modelli marini

High-frequency ultrasound in orthotopic pancreatic cancer models

High-frequency ultrasound in Power Doppler Mode (Vevo 2100 imaging system, FujiFilm VisualSonics, thanks to the help of Dr. Dieter Fuchs) was carried out to evaluate tumor vascularization.
This high-resolution ultrasound instrument provided a non-invasive anatomical image of tumors or normal abdominal organs with an axial resolution of 40 μm, allowing multiple focal depths and improved feature detection. In particular, we observed hypovascular pancreatic tumors below 900 μm in diameter.

This video has been published as Supplemental file to the article:

Avan A, Caretti V, Funel N, Galvani E, Maftouh M, Honeywell RJ, Lagerweij T, Van Tellingen O, Campani D, Fuchs D, Verheul HM, Schuurhuis GJ, Boggi U, Peters GJ, Würdinger T, Giovannetti E.
Crizotinib inhibits metabolic inactivation of gemcitabine in c-Met-driven pancreatic carcinoma
Cancer Res. 2013 Nov 15;73(22):6745-56
Link: www.ncbi.nlm.nih.gov/pubmed/24085787

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Laser-microdissection of pancreatic ductal adenocarcinoma tissues

Example of Laser-microdissection of pancreatic ductal adenocarcinoma tissues using the LMD7000 instrument (Leica Microsystems, Wetzlar, Germany, thanks to the help of Dr. Mauro Baron). The precision of the narrow focus of the laser beam allowed to pickup specimens with high degree of accuracy and extremely low risk of contamination.

This video has been published as supplemental file to the article:

Giovannetti E, Funel N, Peters GJ, Del Chiaro M, Erozenci LA, Vasile E, Leon LG, Pollina LE, Groen A, Falcone A, Danesi R, Campani D, Verheul HM, Boggi U.
MicroRNA-21 in pancreatic cancer: correlation with clinical outcome and pharmacologic aspects underlying its role in the modulation of gemcitabine activity
Cancer Res. 2010;70:4528-38
Link: www.ncbi.nlm.nih.gov/pubmed/20460539

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Primary pancreatic cancer cell cultures

These cells and the methodology used for their isolation have been described in the article:

Funel N, Giovannetti E, Del Chiaro M, Mey V, Pollina LE, Nannizzi S, Boggi U, Ricciardi S, Del Tacca M, Bevilacqua G, Mosca F, Danesi R, Campani D.
Laser microdissection and primary cell cultures improve pharmacogenetic analysis in pancreatic adenocarcinoma
Lab Invest. 2008 Jul;88(7):773-84.
Link: www.ncbi.nlm.nih.gov/pubmed/18490900

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